Integration of Irradiation with Cytoplasmic Incompatibility in the Management of the Pulse Beetle Callosobruchus spp. (Coleoptera: Bruchidae)

Abstract

In the thesis, the impact of radiation on successive generations and cytoplasmic incompatibility of Callosobruchus spp. is studied. C. chinensis and C. maculatus are considered as the study insects. Experiments of oviposition preference and mating competitiveness are also conducted. Study showed that C. chinensis collected either from lentil or Bengal gram preferred lentil << Bengal gram < green gram for oviposition. Regarding the adult emergence, their preferred seeds are green gram > > Bengal gram> Lentil. C. maculatus preferred seed according as they collected from. The highest emergences rate is found in green gram. C. maculatus preferred pea for oviposition but the emergence rate is nearer to zero in it. Irradiation is able to increase the development time of egg to adult in successive generations of C. maculatus. The dose 12Gy the maturity time in F5 generation has gone · more than 100 days. In breeding of the progeny of the irradiated parent do not affect the percentage of adult emergence. For C. chinensis the degree CVs decreases as the treated males increased. Further increase of the number of males the CVs would be the same. For C. maculatus the ratio 1: 1: 1 showed the highest competitiveness values for both radiation doses 6Gy and 8Gy. The dose with 8Gy showed the highest competitiveness value. For 6Gy dose the competitiveness values were the maximum for the ratio 1:1:3 and 1:1:4 and the minimum number of values is attained at the ratio 1:1:2. Both in C. chinensis and C. maculatus the regional and reciprocal crosses have