Abstract:
The potential of anther culture response of four
commercial barley cultivars: Abbey, Dallas, Bianka and
Decado was investigated on maltose and sucrose-based
media. Although there was difference in response among
the genotypes the substitution of sucrose by maltose led
to an improvement in anther response as well as in green
plant regeneration. Higher concentrations of maltose
( 6-9% w/v) favoured regeneration via embryogenetic route.
In the study of transmission of anther culture
responsiveness into hybrids produced from crosses between
Hordeum vulgare cv. Prisma (P) and four H. spontaneum
lines (HS2, HS10, HS16 and HS20), it was found that,
despite the improvement obtained using maltose, anther
culture response was largely dependent on the ge~otype.
A heterotic effect was also observed in the Fl hybrids.
Results presented in this study also focused on the
development, evaluation and exploitation of genetic
markers in barley. Both protein (a-amylase, water soluble
protein, grain esterase, leaf esterase and hordein)
and molecular ( RAPD} markers were used as a means of
detecting polymorphism in H. vulgare cv. Prisma and four
H. spontaneum lines (HS2, HS10. HS16 and HS20}. Except
hordein, all other markers detected polymorphism between
H. vulgare and H. spontaneum. These polymorphic markers
showed segregation in the five doubled haploids derived
from PXHS2 hybrid.-----
Description:
This Thesis is Submitted to the Department of Botany, University of Rajshahi, Rajshahi, Bangladesh for The Degree of Doctor of Philosophy (PhD)