Abstract:
In an attempt to develop seedless Momordica dioica Roxb., diploid, triploid
and tetraploid genotypes having chromosome numbers 2n=28, 3n=42 and 4n=56
respectively were treated with aqueous colchicine solution of different
concentrations namely, 0.1, 0.2, 0.3, 0.4, 0.5, 0:1 and 1.0% colchicine was applied
with different methods viz., dipping, dropping and immersed on seeds and 2, 4 and
6 days old seedling for the induction of artificial polyploid type.
Abnormal seedling growth, reduction in germination and significant delay in
germination time were recorded, when both duration of treatment time and
concentration of colchicine were gradually increased. Affected percentage was
very high in the dropping method in 4 and 6 days old seedling when higher
concentration and long treatment time were given. Mortality percentage was very
high when the shoot tips were immersed for long time, and no plant was found to
survive when seedlings were dipped in any strength of colchicine solution for 24h.
From the affected seeds, ------
Description:
This Thesis is Submitted to the Department of Botany, University of Rajshahi, Rajshahi, Bangladesh for The Degree of Master of Philosophy (MPhil)